Review



yutaka hata  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc yutaka hata
    Yutaka Hata, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yutaka+hata/pClneoMyc+human+ERBIN+(Plasmid+%2340214)/bio_rxiv__2025__06__13__659557-213-28-30
    Average 93 stars, based on 2 article reviews
    yutaka hata - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Inflammasome Activation in Cutaneous Squamous Cell Carcinoma
    Article Snippet: The pBabe-KrasG12D, pQCXIP-mCherry-Halo-YAP1, and mCherry2-C1 plasmids were acquired from Addgene (Watertown, MA). pBabe-Kras G12D was a gift from Channing Der (Addgene plasmid # 58902; http://n2t.net/addgene:58902 ; RRID:Addgene_58902). .. The pQCXIP-mCherry-Halo-YAP1 plasmid was a gift from Yutaka Hata (Addgene plasmid # 128336; http://n2t.net/addgene:128336 ; RRID:Addgene_128336) . mCherry2-C1 was a gift from Michael Davidson (Addgene plasmid # 54563; http://n2t.net/addgene:54563 ; RRID:Addgene_54563). ..

    Article Title: The histone chaperone ANP32B regulates chromatin incorporation of the atypical human histone variant macroH2A.
    Article Snippet: .. We thank the Histone Source at Colorado State University for the purification of human histones. pCAGGS-ANP32B was a kind gift fromWendy Barclay and pET29aYS14 was a kind gift from Jung-Hyun Min (Addgene plasmid # 66890). pQCXIP-GFP1-10 was a gift from Yutaka Hata (Addgene plasmid # 68715) and pRK-flag-GFP11 from Yihong Ye (Addgene plasmid # 78590). ..

    Article Title: Mechano-signaling of prostate tumor initiating cells facilitates their tropism to stiff metastatic niche
    Article Snippet: .. Short hairpin RNA (shRNA) constructs were obtained from Sigma’s MISSION library (Kindly provided by Department of Molecular Cell Biology, LUMC). pDEST/Lifeact-mCherry-N1 was a gift from Robin Shaw (Addgene plasmid # 40908), pmCherry Paxillin was a gift from Kenneth Yamada (Addgene plasmid # 50526) and pmCherry Paxillin (#50526) and pLL3.7 EGFPC2 TAZ was a gift from Yutaka Hata (Addgene plasmid # 66850). ..

    Article Title: Active Merlin Binds RalB to Regulate Exocytosis
    Article Snippet: The plasmids expressing GFP fusions for a-Catenin, d-catenin, PPP1R12A, RhoA, RALA and RALB cDNA ORF Clones cDNA ORF Clone, were purchased from Sino Biological US, Inc. (Wayne, PA, Cat#: HG-12388-ACG, HG-16488-ACG, HG-13990-ACG, HG12110-ANG, HG52476-ANG, HG15052-ANG). .. The plasmids pEGFP-C3-Exo70, pEGFP-C3-Sec8, pEGFP-C3-Exo84 and pEGFP-C3-Sec5 were a gift from Channing Der. (Addgene plasmids # 53761, # 53758, #53762, #53756. pClneoMyc human ERBIN was a gift from Yutaka Hata (Addgene plasmid # 40214. .. Full-length MLCK-GFP was a gift from Anne Bresnick (Addgene plasmid # 46316. pcDNA3-EGFP-Cdc42(wt) was a gift from Klaus Hahn (Addgene plasmid # 12599).

    Article Title: Coexisting mechanisms of luminogenesis in pancreatic cancer-derived organoids
    Article Snippet: The PCR reactions were performed with the Platinum SuperFi Green PCR Mastermix (Invitrogen 12369050) as described in the manufacturer protocol with the following plasmids and primers. .. The CMV promoter was extracted from mRuby-LC-Myosin-N-7,a gift from Michael Davidson (Addgene plasmid # 55871), the myosinIIA-GFP from Myosin-IIA-GFP was a gift from Matthew Krummel (Addgene plasmid # 38297), the IRES sequence from pQCXIP-mCherry-Halo-YAP1 was a gift from Yutaka Hata (Addgene plasmid # 128336), and the Neomycin resistance from mRuby-LC-Myosin-N-7 was a gift from Michael Davidson (Addgene plasmid # 55871). .. The PCR primers were used: CMV promoter: Forward 5’-GGAGTTCCGCGTTACATAACTTACG-3’ Reverse 5’-GTTCACTAAACCAGCTCTGCTTATAT-3’ Myosin-IIA-GFP: Forward 5’-CTACCGGTCGCCACCATGG-3’ Reverse 5’-CTGATTATGATCAGTTATCTAGAAGCG-3’ IRES: Forward 5’-CCGTCTTTTGGCAATGTGAGGG-3’ Reverse 5’-TTATCATCGTGTTTTTCAAAGGAAA-3’ Neomycin: Forward 5’-ATGATTGAACAAGATGGATTGCACGC-3’ Reverse 5’-CCCCAGAGTCCCGCTCAGAAG-3’ DNA fragments were separated by gel electrophoresis using a 1% agarose gel (Roth 3810.3).

    Article Title: Coexisting mechanisms of luminogenesis in pancreatic cancer-derived organoids
    Article Snippet: MyosinIIA-GFP , Matthew Krummel , Plasmid #38297, http://n2t.net/addgene:38297 , RRID: Addgene_38297. .. pQCXIP-mCherry-Halo-YAP1 , Yutaka Hata , Plasmid #128336, http://n2t.net/addgene:128336 , RRID: Addgene_128336. ..

    Article Title: Structural basis of TMPRSS2 zymogen activation and recognition by the HKU1 seasonal coronavirus
    Article Snippet: .. Codon-optimized synthetic genes coding for the full-length spike of HKU1 B/C isolate N5P8 (referred to as HKU1B, UniProtKB/Swiss-Prot: Q0ZME7.1) and those coding for TMPRSS2 from mouse (Mouse C57BL/6 - UniPropt: Q3UKE3), ferret (Ferret – UniProt: A0A8U0SMZ2), hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank: AJ318514) by replacing the VSV-G gene. pQCXIP-Empty control plasmid was previously described . pQCXIP-BSR-GFP11 and pQCXIP-GFP1-10 were a kind gift from Yutaka Hata (Addgene plasmid #68716 and #68715). pCSDest-TMPRSS2 was a kind gift from Roger Reeves (Addgene plasmid # 53887). ..

    Purification:

    Article Title: The histone chaperone ANP32B regulates chromatin incorporation of the atypical human histone variant macroH2A.
    Article Snippet: .. We thank the Histone Source at Colorado State University for the purification of human histones. pCAGGS-ANP32B was a kind gift fromWendy Barclay and pET29aYS14 was a kind gift from Jung-Hyun Min (Addgene plasmid # 66890). pQCXIP-GFP1-10 was a gift from Yutaka Hata (Addgene plasmid # 68715) and pRK-flag-GFP11 from Yihong Ye (Addgene plasmid # 78590). ..

    Transfection:

    Article Title: Influenza A virus NS1 suppresses nuclear speckles promoted gene expression by inhibition of transcription.
    Article Snippet: .. Cells, plasmids, viruses, chemicals and antibodies, siRNAs Reporter plasmids CMV-LUC2CP/ARE and CMV-LUC2CP/intron/ARE (GideonDreyfuss, addgene #62857 and #62858), pQCXIP-GFP1-10 (gift of npj Viruses | (2025) 3:46 8 Yutaka Hata, addgene #68715), and pCW57.1-luc (gift from Stephen Tapscott, addgene #99283), pSBtet rtTA G72V SE Δsplice GP Luc (gift of AlexanderMcLellan addgene #194328), SB100X in pCAGglobin pA (gift of Mark Groudine, addgene #127909) were obtained from Addgene. pSV40luc. pCMV-luc and pCMV-RlucpA-IRES-FlucpA were described earlier4. mCherry2-SRRM1 was used as a source for the nuclear speckle localising domain of SRRM1 and was obtained from Addgene (#54563). pEGFPCyclin L1was used as a source for the Cyclin L1 nuclear speckle localisation domain (gift of W. Becker, Aachen) HEK293 (transfections), A549 (RNASeq, infections), or MDCK (split gfp assays) cells were used throughout this study. ..

    RNA sequencing:

    Article Title: Influenza A virus NS1 suppresses nuclear speckles promoted gene expression by inhibition of transcription.
    Article Snippet: .. Cells, plasmids, viruses, chemicals and antibodies, siRNAs Reporter plasmids CMV-LUC2CP/ARE and CMV-LUC2CP/intron/ARE (GideonDreyfuss, addgene #62857 and #62858), pQCXIP-GFP1-10 (gift of npj Viruses | (2025) 3:46 8 Yutaka Hata, addgene #68715), and pCW57.1-luc (gift from Stephen Tapscott, addgene #99283), pSBtet rtTA G72V SE Δsplice GP Luc (gift of AlexanderMcLellan addgene #194328), SB100X in pCAGglobin pA (gift of Mark Groudine, addgene #127909) were obtained from Addgene. pSV40luc. pCMV-luc and pCMV-RlucpA-IRES-FlucpA were described earlier4. mCherry2-SRRM1 was used as a source for the nuclear speckle localising domain of SRRM1 and was obtained from Addgene (#54563). pEGFPCyclin L1was used as a source for the Cyclin L1 nuclear speckle localisation domain (gift of W. Becker, Aachen) HEK293 (transfections), A549 (RNASeq, infections), or MDCK (split gfp assays) cells were used throughout this study. ..

    shRNA:

    Article Title: Mechano-signaling of prostate tumor initiating cells facilitates their tropism to stiff metastatic niche
    Article Snippet: .. Short hairpin RNA (shRNA) constructs were obtained from Sigma’s MISSION library (Kindly provided by Department of Molecular Cell Biology, LUMC). pDEST/Lifeact-mCherry-N1 was a gift from Robin Shaw (Addgene plasmid # 40908), pmCherry Paxillin was a gift from Kenneth Yamada (Addgene plasmid # 50526) and pmCherry Paxillin (#50526) and pLL3.7 EGFPC2 TAZ was a gift from Yutaka Hata (Addgene plasmid # 66850). ..

    Construct:

    Article Title: Mechano-signaling of prostate tumor initiating cells facilitates their tropism to stiff metastatic niche
    Article Snippet: .. Short hairpin RNA (shRNA) constructs were obtained from Sigma’s MISSION library (Kindly provided by Department of Molecular Cell Biology, LUMC). pDEST/Lifeact-mCherry-N1 was a gift from Robin Shaw (Addgene plasmid # 40908), pmCherry Paxillin was a gift from Kenneth Yamada (Addgene plasmid # 50526) and pmCherry Paxillin (#50526) and pLL3.7 EGFPC2 TAZ was a gift from Yutaka Hata (Addgene plasmid # 66850). ..

    Sequencing:

    Article Title: Coexisting mechanisms of luminogenesis in pancreatic cancer-derived organoids
    Article Snippet: The PCR reactions were performed with the Platinum SuperFi Green PCR Mastermix (Invitrogen 12369050) as described in the manufacturer protocol with the following plasmids and primers. .. The CMV promoter was extracted from mRuby-LC-Myosin-N-7,a gift from Michael Davidson (Addgene plasmid # 55871), the myosinIIA-GFP from Myosin-IIA-GFP was a gift from Matthew Krummel (Addgene plasmid # 38297), the IRES sequence from pQCXIP-mCherry-Halo-YAP1 was a gift from Yutaka Hata (Addgene plasmid # 128336), and the Neomycin resistance from mRuby-LC-Myosin-N-7 was a gift from Michael Davidson (Addgene plasmid # 55871). .. The PCR primers were used: CMV promoter: Forward 5’-GGAGTTCCGCGTTACATAACTTACG-3’ Reverse 5’-GTTCACTAAACCAGCTCTGCTTATAT-3’ Myosin-IIA-GFP: Forward 5’-CTACCGGTCGCCACCATGG-3’ Reverse 5’-CTGATTATGATCAGTTATCTAGAAGCG-3’ IRES: Forward 5’-CCGTCTTTTGGCAATGTGAGGG-3’ Reverse 5’-TTATCATCGTGTTTTTCAAAGGAAA-3’ Neomycin: Forward 5’-ATGATTGAACAAGATGGATTGCACGC-3’ Reverse 5’-CCCCAGAGTCCCGCTCAGAAG-3’ DNA fragments were separated by gel electrophoresis using a 1% agarose gel (Roth 3810.3).

    Clone Assay:

    Article Title: Structural basis of TMPRSS2 zymogen activation and recognition by the HKU1 seasonal coronavirus
    Article Snippet: .. Codon-optimized synthetic genes coding for the full-length spike of HKU1 B/C isolate N5P8 (referred to as HKU1B, UniProtKB/Swiss-Prot: Q0ZME7.1) and those coding for TMPRSS2 from mouse (Mouse C57BL/6 - UniPropt: Q3UKE3), ferret (Ferret – UniProt: A0A8U0SMZ2), hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank: AJ318514) by replacing the VSV-G gene. pQCXIP-Empty control plasmid was previously described . pQCXIP-BSR-GFP11 and pQCXIP-GFP1-10 were a kind gift from Yutaka Hata (Addgene plasmid #68716 and #68715). pCSDest-TMPRSS2 was a kind gift from Roger Reeves (Addgene plasmid # 53887). ..

    Control:

    Article Title: Structural basis of TMPRSS2 zymogen activation and recognition by the HKU1 seasonal coronavirus
    Article Snippet: .. Codon-optimized synthetic genes coding for the full-length spike of HKU1 B/C isolate N5P8 (referred to as HKU1B, UniProtKB/Swiss-Prot: Q0ZME7.1) and those coding for TMPRSS2 from mouse (Mouse C57BL/6 - UniPropt: Q3UKE3), ferret (Ferret – UniProt: A0A8U0SMZ2), hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank: AJ318514) by replacing the VSV-G gene. pQCXIP-Empty control plasmid was previously described . pQCXIP-BSR-GFP11 and pQCXIP-GFP1-10 were a kind gift from Yutaka Hata (Addgene plasmid #68716 and #68715). pCSDest-TMPRSS2 was a kind gift from Roger Reeves (Addgene plasmid # 53887). ..



    Similar Products

    93
    Addgene inc yutaka hata
    Yutaka Hata, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yutaka+hata/pClneoMyc+human+ERBIN+(Plasmid+%2340214)/bio_rxiv__2025__06__13__659557-213-28-30
    Average 93 stars, based on 1 article reviews
    yutaka hata - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    94
    Addgene inc yutaka hata 47
    Yutaka Hata 47, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yutaka+hata/pQCXIP-GFP1-10+(Plasmid+%2368715)/pmc11331971-357-54-57
    Average 94 stars, based on 1 article reviews
    yutaka hata 47 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results